ʻO ka Pepstatin kahi pentapeptide, kahi mea hoʻopiʻi aspartyl protease inhibitor, hiki ke pale i ka protease aspartic a me ka protease acid o nā microorganisms like ʻole.ʻO ka Pepstatin ka mea huna e nā ʻano Streptomyces a hana ʻia e Streptomyces.Hiki iā ia ke kāohi i ka pepsin, pepsin D a me ka angiotensin-releasing enzyme, a loaʻa nā hopena therapeutic i ka maʻi ʻōpū o ka ʻōpū, ke kiʻekiʻe kiʻekiʻe o ka renal, ka arthritis, carrageenan edema a me nā maʻi ʻē aʻe.
ʻO Pepstatin kahi mea paʻa ikaika o nā protease aspartyl e like me pepsin, cathepsin D, a me renin.ʻO kēia pentapeptide maoli i hoʻokaʻawale ʻia mai nā actinomycetes no nā makahiki he nui ka mea hoʻopaneʻe renin maʻamau i vitro. ʻAʻole kikoʻī ʻo Pepstatin no ka renin a ʻaʻole hiki ke hoʻoheheʻe ʻia i ka wai.Ua hoʻonui ʻia nā derivatives Structural o ka pepstatin i kona solubility a me ka kikoʻī no ka renin e kekahi mau kauoha o ka magnitude. Aia ka Pepstatin i ka γ amino acid statin maʻamau e hiki ke hoʻololi i nā waikawa amino ʻelua ma ka paʻa scissile o ka substrate protein a me ka wehe ʻana o ka substrate ma muli o ke ʻano hoʻohālikelike. i kahi kūlana hoʻololi o ka peptide bond hydrolysis e aspartyl protease.
Ke hoʻokuʻu ʻia ka protein mai nā cell haʻihaʻi, hiki ke hoʻokuʻu ʻia nā proteases, pono e hoʻopaʻa koke ʻia i mea e hoʻohaʻahaʻa ʻia ai ka protein.I ke kaʻina hana o ka hoʻoneʻe ʻana i ka protein, pono e hoʻohui ʻia nā protease inhibitors e pale ai i ka proteolysis.ʻO ka mea hoʻopaneʻe protease e pili ana i kahi mea e hoʻopaʻa ai i kekahi mau pūʻulu ma ke kikowaena ikaika o nā molekele protease, no laila e emi a nalo paha ka hana protease, akā ʻaʻole ia e denature i ka protein enzyme.ʻOkoʻa ka naʻau o nā protease like ʻole i nā protein like ʻole, no laila pono e hoʻoponopono i ka ʻike o nā protease like ʻole.Ma muli o ka haʻahaʻa loa o ka solubility o ka protease inhibitor i loko o ka wai, pono e uku nui ʻia i kēlā protease inhibitor pono e hui piha ʻia i loko o ka pale e hoʻemi i ka ua o ka protease inhibitor.Hiki i ka Pepstantin A ke pale i nā protease waikawa e like me pepsin, angiotensin, collagenase, cathepsin D a me chymosin.
ʻO ka Pepstatin A kahi mea hoʻokae o ka cathepsin d a me ka e.Ma hope o ka mālamaʻiaʻana o nā pūnaewele HEK293 me nā manaʻo likeʻole o ka pepstatin A no nā hola 24, uaʻikeʻia ka'ōlelo o LC3Ⅱ a me p62.Ua hōʻike nā hopena i hiki i ka pepstatin A ke hoʻonui nui i ka hōʻike o LC3Ⅱ a me P62 (P<0.05) ma ke ʻano pili i ka dose.Ua hoʻohanaʻia ka 20μ g / ml pepstatin A no ka mālamaʻana iā HEK293 i nā manawa likeʻole, a uaʻikeʻia nā hopena o nā manawa likeʻole ma ka'ōlelo o LC3II a me p62.Ua hōʻike ʻia nā hopena e hiki i ka pepstatin A ke hoʻoponopono i ka ʻōlelo o LC3II a me p62 i ke ʻano o ka manawa.
He mea hana polypeptide mākou ma Kina, me nā makahiki he nui o ka ʻike makua i ka hana polypeptide.ʻO Hangzhou Taijia Biotech Co., Ltd. he mea hana ʻoihana polypeptide raw material, hiki ke hāʻawi i nā ʻumi kaukani o ka polypeptide raw material a hiki ke hana ʻia e like me nā pono.He maikaʻi ka maikaʻi o nā huahana polypeptide, a hiki i ka maʻemaʻe ke hiki i ka 98%, iʻikeʻia e nā mea hoʻohana ma ka honua holoʻokoʻa.Welcome e nīnau iā mākou.
