ʻO GsMTx4 he peptide 35-amino acid me ʻehā mau paʻa disulfide i hana ʻia i ke kumu o ka cysteine knot, kahi hiʻohiʻona maʻamau o nā peptides spider venom e hāʻawi i ke kūpaʻa a me ka kikoʻī.ʻAʻole i wehewehe piha ʻia ke ʻano o ka hana o GsMTx4, akā manaʻo ʻia ua hoʻopaʻa ʻia i nā kikowaena extracellular a transmembrane o cationic MSCs a hoʻopaʻa i ko lākou pore wehe a i ʻole ka puka ʻana ma o ka hoʻololi ʻana i kā lākou conformation a i ʻole ke ʻano o ka membrane.Ua hōʻike ʻia ʻo GsMTx4 e kāohi i kekahi mau MSC cationic me nā koho like ʻole a me ka mana.No ka laʻana, GsMTx4 inhibits TRPC1 me kahi IC50 o 0.5 μM, TRPC6 me kahi IC50 o 0.2 μM, Piezo1 me kahi IC50 o 0.8 μM, Piezo2 me kahi IC50 o 0.3 μM, akā ʻaʻohe hopena ma TRPV1 a i ʻole TRPV1. μM.(Bae C et al 2011, Biochemistry)
Ua hoʻohana ʻia ʻo GsMTx4 ma ke ʻano he lāʻau lapaʻau e aʻo ai i ka hana a me ka hoʻoponopono ʻana o nā MSC cationic i nā ʻano cell a me nā kiko.ʻO kekahi o nā laʻana:
Hiki i ka GsMTx4 ke ālai i nā MSC i hoʻāla ʻia e ka hoʻohola ʻana i loko o nā astrocytes, nā puʻuwai puʻuwai, nā ʻiʻo ʻiʻo maʻemaʻe a me nā ʻiʻo ʻiʻo iwi.ʻO nā Astrocytes he mau pūnana hoku e kākoʻo ana i ka lolo a me ke kuamoʻo.ʻO nā puʻuwai puʻuwai nā pūnaewele e hana ai i ka ʻiʻo puʻuwai.ʻO nā ʻiʻo ʻiʻo maʻemaʻe nā cell e hoʻomalu i ka neʻe ʻana o nā kino e like me ka ʻōpū a me nā kīʻaha koko.ʻO nā ʻiʻo ʻiʻo iwi ʻiʻo nā pūnaewele e hiki ai i ka neʻe ʻana o ke kino.Ma ka pale ʻana i nā MSC i loko o kēia mau pūnaewele, hiki i ka GsMTx4 ke hoʻololi i ko lākou mau waiwai uila, nā pae calcium, ka ʻokiʻoki a me ka hoʻomaha, a me ka hōʻike ʻana i ke ʻano.Hiki i kēia mau hoʻololi ke hoʻololi i ka hana maʻamau o kēia mau pūnaewele a i ʻole nā maʻi maʻi (Suchyna et al., Nature 2004; Bae et al., Biochemistry 2011; Ranade et al., Neuron 2015; Xiao et al., Nature Chemical Biology 2011)
Hiki iā GsMTx4 ke kāohi i kahi ʻano MSC kūikawā i kapa ʻia ʻo TACAN, kahi i komo i ka pane ʻeha.ʻO TACAN kahi ala i hōʻike ʻia i loko o nā pūnana nerve e ʻike ai i ka ʻeha.Hoʻohana ʻia ʻo TACAN e nā mea hoʻoikaika mechanical, e like me ke kaomi ʻana a i ʻole ke kuʻi ʻana, a hoʻoulu i ka ʻeha.Hiki i ka GsMTx4 ke hoʻemi i ka hana o TACAN a hoʻemi i ka hana ʻeha i nā ʻano holoholona o ka ʻeha mechanical (Wetzel et al., Nature Neuroscience 2007; Eijkelkamp et al., Nature Communications 2013)
Hiki iā GsMTx4 ke pale aku i nā astrocytes mai nā mea ʻawaʻawa i hoʻokomo ʻia e kahi mole i kapa ʻia ʻo lysophosphatidylcholine (LPC), ʻo ia ka lipid mediator e hōʻeha ai i ka lolo a me ke kuamoʻo.Hiki i ka LPC ke hoʻoikaika i nā MSC i nā astrocytes a hoʻoulu iā lākou i ka nui o ka calcium, kahi e alakaʻi ai i ke kaumaha oxidative a me ka make cell.Hiki iā GsMTx4 ke pale i ka LPC mai ka hoʻoulu ʻana i nā MSC i nā astrocytes a pale iā lākou mai nā mea ʻona.Hiki i ka GsMTx4 ke hōʻemi i ka pōʻino o ka lolo a hoʻomaikaʻi i ka hana neurological i nā ʻiole i hoʻopaʻa ʻia me LPC (Gottlieb et al., Journal of Biological Chemistry 2008; Zhang et al., Journal of Neurochemistry 2019)
Hiki i ka GsMTx4 ke hoʻololi i ka hoʻokaʻawale ʻana o ka neural stem cell ma ke kāohi ʻana i kahi ʻano MSC kikoʻī i kapa ʻia ʻo Piezo1, i hōʻike ʻia i loko o nā pūnaewele neural stem.ʻO nā cell stem neural nā pūnaewele e hiki ke hana i nā neurons hou a i ʻole nā ʻano ʻano o ka lolo.ʻO Piezo1 kahi ala i hoʻāla ʻia e nā ʻike mechanical mai ke kaiapuni, e like me ka ʻoʻoleʻa a i ʻole ke kaomi ʻana, a hoʻololi i ka hoʻoholo ʻana o nā cell stem neural i ke ʻano o ke kelepona e lilo ai.Hiki i ka GsMTx4 ke hoʻopilikia i ka hana Piezo1 a hoʻololi i ka ʻokoʻa o ka neural stem cell mai nā neurons i astrocytes (Pathak et al., Journal of Cell Science 2014; Lou et al., Cell Reports 2016)
He mea hana polypeptide mākou ma Kina, me nā makahiki he nui o ka ʻike makua i ka hana polypeptide.ʻO Hangzhou Taijia Biotech Co., Ltd. he mea hana ʻoihana polypeptide raw material, hiki ke hāʻawi i nā ʻumi kaukani o ka polypeptide raw material a hiki ke hana ʻia e like me nā pono.He maikaʻi ka maikaʻi o nā huahana polypeptide, a hiki i ka maʻemaʻe ke hiki i ka 98%, iʻikeʻia e nā mea hoʻohana ma ka honua holoʻokoʻa.Welcome e nīnau iā mākou.
